Human IL-10 T cell ELISPOT kit

IL-10 specific spots produced by 5x10E4 human PBMC.
Stimulation: PMA/ionomycin.

Catalogue Number

CT235-T2

Description

Human IL-10 T cell ELISPOT kit - 2-plate

Price

€330.00

staining

Silver

Characteristics

Transparent

Contents

coating antibodies
biotinylated detector antibodies
phi-labeled goat anti-biotin antibodies
activator solutions
Blocking stock solution
Dilution buffer T
Tween-20
transparent ELISPOT plates with lids and cover slips
detailed manual

Documentation

References list

Metabolic and immune effects of immunotherapy with proinsulin peptide in human new-onset type 1 diabetes.
Alhadj Ali, Mohammad, et al. Sci Transl Med 2017 Aug 09;9:
Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health.
Arif, Sefina, et al. J. Clin. Invest. 2004 Feb;113: 451-63
Self-glycolipids modulate dendritic cells changing the cytokine profiles of committed autoreactive T cells.
Buschard, Karsten, et al. PLoS ONE 2012;7: e52639
Development of type 1 diabetes despite severe hereditary B-cell deficiency.
Martin, S, et al. N. Engl. J. Med. 2001 10 04;345: 1036-40
Development of novel LL-37 derived antimicrobial peptides with LPS and LTA neutralizing and antimicrobial activities for therapeutic application.
Nell, Marja J, et al. Peptides 2006 Apr;27: 649-60
Beta-cell function in new-onset type 1 diabetes and immunomodulation with a heat-shock protein peptide (DiaPep277): a randomised, double-blind, phase II trial.
Raz, I, et al. Lancet 2001 Nov 24;358: 1749-53
Islet antigen specific IL-10+ immune responses but not CD4+CD25+FoxP3+ cells at diagnosis predict glycemic control in type 1 diabetes.
Sanda, Srinath, et al. Clin. Immunol. 2008 May;127: 138-43
CD8 T-cell reactivity to islet antigens is unique to type 1 while CD4 T-cell reactivity exists in both type 1 and type 2 diabetes.
Sarikonda, Ghanashyam, et al. J. Autoimmun. 2014 May;50: 77-82
Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.
Schloot, Nanette C, et al. J. Autoimmun. 2003 Dec;21: 365-76
Redirection of human autoreactive T-cells Upon interaction with dendritic cells modulated by TX527, an analog of 1,25 dihydroxyvitamin D(3).
van Halteren, Astrid G S, et al. Diabetes 2002 Jul;51: 2119-25
Autoantibodies and associated T-cell responses to determinants within the 831-860 region of the autoantigen IA-2 in Type 1 diabetes.
Weenink, S M, et al. J. Autoimmun. 2009 Sep;33: 147-54
Human mesenchymal stem cells modulate cellular immune response to islet antigen glutamic acid decarboxylase in type 1 diabetes.
Zanone, Maria M, et al. J. Clin. Endocrinol. Metab. 2010 Aug;95: 3788-97