Human IgG B cell ELISPOT kit - 5-plate
SKU
CT780-B5
Complete ELISPOT kit including all reagents (480 determinations).
Format
5-plate
Price
€ 670
Documentation
Reactivity
Human
Category
B cell ELISPOT
Analyte
IgG
Description
Our B cell ELISPOT kits for determining the frequency of IgG-secreting B cells provide a comprehensive, ready-to-use solution for accurate, high-performance ELISPOT assay.
Each kit includes all essential components: coating and detection antibodies, B cell stimulus, streptavidin-HRP conjugate, TMB substrate, blocking stock solution, dilution buffer, and Tween-20.
Each kit includes all essential components: coating and detection antibodies, B cell stimulus, streptavidin-HRP conjugate, TMB substrate, blocking stock solution, dilution buffer, and Tween-20.
Analyte information
Description
We offer high-performance B cell ELISPOT kits for detection of antibody secreting cells (ASCs) in human, monkey and mouse samples, including blood or lymphoid tissue.
The B cells ELISPOT is a powerful tool to determine antigen-specific humoral immunity by identifying individual memory B cells and plasma cells. While traditional ELISA assays quantify circulating antibodies and it does not capture the full immune response driven by long-lived memory B cells. The B cell ELISPOT overcomes this limitation by enabling ex vivo activation and detection of antigen-specific memory B cells at the single cell level. Antigen-specific immunoglobulin-secreting B cells can be analyzed either by coating plates with antigen or by using biotinylated antigen in the detection step.
This assay is widely recognized for monitoring vaccine induced memory and immune memory. It allows researchers to assess both the strength and durability of immune protection following vaccination or infection.
The B cells ELISPOT is a powerful tool to determine antigen-specific humoral immunity by identifying individual memory B cells and plasma cells. While traditional ELISA assays quantify circulating antibodies and it does not capture the full immune response driven by long-lived memory B cells. The B cell ELISPOT overcomes this limitation by enabling ex vivo activation and detection of antigen-specific memory B cells at the single cell level. Antigen-specific immunoglobulin-secreting B cells can be analyzed either by coating plates with antigen or by using biotinylated antigen in the detection step.
This assay is widely recognized for monitoring vaccine induced memory and immune memory. It allows researchers to assess both the strength and durability of immune protection following vaccination or infection.
Alternative names
IgG, Immunoglobulin G
Gene ID
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