Inhibits the proteolytic degradation of cytokines by serum and plasma proteases. For ELISA assays, add an appropriate amount of Cytokine Stabilization Buffer to serum or plasma samples before analysis.
TMB solution contains 3,3′,5,5′-tetramethylbenzidine, the substrate for horseradish peroxidase (HRP) in our ELISA kits, enabling colorimetric detection and quantification.
TMB solution contains 3,3′,5,5′-tetramethylbenzidine, the substrate for horseradish peroxidase (HRP) in our ELISPOT kits, enabling spot detection and quantification.
Tween-20 is a non-ionic detergent used in ELISA and ELISPOT assays to reduce non-specific binding of proteins and immunoglobulins to the plate surface while preserving specific antibody-cytokine interactions.