AEC | 3-amino-9-ethylcarbazole |
APC | Antigen presenting cell |
ASC | Antibody secreting cells |
BSA | Bovine Serum Albumin |
CD | Cluster of Differentiation |
CNS | Central Nerve System |
ConA | Concanavalin A |
CSB | Cytokine stabilization buffer |
CTL | Cytotoxic T Lymphocytes |
DMSO | Dimethyl sulfoxide |
ELISA | Enzyme-linked immunosorbent assay |
ELISPOT | Enzyme-linked immunosorbent spot assay |
EPO | Erythropoietin |
FCS | Fetal calf serum |
GABA | Phi-labeled anti-biotin antibodies |
G-CSF | Granulocyte colony stimulating factor |
GM-CSF | Granulocyte-macrophage colony stimulating factor |
HI-FCS | Heat-inactivated (20' 56°C) FCS |
HRP | Horseradish Peroxidase |
IFN | Interferon |
Ig | Immunoglobulin |
IL | Interleukin |
LPS | Lipopolysaccharide |
MHC | Major Histocompatibiliy Complex |
nat | natural |
OD | Optical density |
PBMC | Peripheral Blood Mononuclear Cells |
PBS | Phosphate-buffered saline |
PBS-I | Commercially available sterile and pyrogen free PBS |
PBST | PBS containing 0.05% Tween-20 |
PBST-B | PBS containing 0.05% Tween-20 and 0.5% BSA |
PC | Producing cell |
PHA | Phytohaemagglutinin |
PMA | Phorbol 12-myristate 13-acetate |
PPD | Purified Protein Derivative (tuberculin) |
PS | Polystyrene |
PVDF | Polyvinylidene fluoride |
Qty | Quantity |
rec | recombinant |
RT | Room temperature |
SFC | Spot forming cell |
SPP | Streptavidin-HRP polymer |
TMB | Tetramethylbenzidine |
TNF | Tumor necrosis factor |
TT | Tetanus toxoid |
VAT | Value added tax |