Abbreviations

AEC 3-amino-9-ethylcarbazole
APC Antigen presenting cell
ASC Antibody secreting cells
BSA Bovine Serum Albumin
CD Cluster of Differentiation
CNS Central Nerve System
ConA Concanavalin A
CSB Cytokine stabilization buffer
CTL Cytotoxic T Lymphocytes
DMSO Dimethyl sulfoxide
ELISA Enzyme-linked immunosorbent assay
ELISPOT Enzyme-linked immunosorbent spot assay
EPO Erythropoietin
FCS Fetal calf serum
GABA Phi-labeled anti-biotin antibodies
G-CSF Granulocyte colony stimulating factor
GM-CSF Granulocyte-macrophage colony stimulating factor
HI-FCS Heat-inactivated (20' 56°C) FCS
HRP Horseradish Peroxidase
IFN Interferon
Ig Immunoglobulin
IL Interleukin
LPS Lipopolysaccharide
MHC Major Histocompatibiliy Complex
nat natural
OD Optical density
PBMC Peripheral Blood Mononuclear Cells
PBS Phosphate-buffered saline
PBS-I Commercially available sterile and pyrogen free PBS
PBST PBS containing 0.05% Tween-20
PBST-B PBS containing 0.05% Tween-20 and 0.5% BSA
PC Producing cell
PHA Phytohaemagglutinin
PMA Phorbol 12-myristate 13-acetate
PPD Purified Protein Derivative (tuberculin)
PS Polystyrene
PVDF Polyvinylidene fluoride
Qty Quantity
rec recombinant
RT Room temperature
SFC Spot forming cell
SPP Streptavidin-HRP polymer
TMB Tetramethylbenzidine
TNF Tumor necrosis factor
TT Tetanus toxoid
VAT Value added tax