ELISPOT in Infectious Diseases

ELISPOT and infectious diseasesThe ELISPOT assay can be particularly helpful in studying infectious diseases, such as HIV, hepatitis, smallpox, schistosomiasis and tuberculosis. Often reliable measurements are needed of immune responses to specific bacterial, parasitic or viral infections. For example, in Mycobacterium tuberculosis qualitative ELISPOT data for specific early secretory antigens offer improved specificity over the purified protein derivative (PPD) skin test reaction in the detection of M. tuberculosis infection. Furthermore, immune responses against pathogens can be monitored through time in a follow-up, to estimate the induction of a substantial memory response in vaccine recipients. One of the advantages of the ELISPOT assay is that it detects single antigen-specific T cells only thereby permitting the direct calculation of responder T cell frequencies. Many researchers study the balance between two CD4+ T cell responses (namely Th1 and Th2), which can be identified by the nature of different and mutual exclusive secreted cytokines. For example, researchers use different ELISPOT assays simultaneously to study antigen-specific T cell responses for Th1 (e.g. IFN-γ and IL-2) and Th2 (e.g. IL-4 and IL-13) cytokines in (non) vaccinated subjects.


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Examples of studies using our ELISPOT assays:

Click on the authors for the abstract of the below mentioned acticles or find them in our Reference Database.

Bai B., Lu X, Meng J, Hu Q, Mao P, Lu B, Chen Z, Yuan Z. and Wang H.
Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses.
Mol. Immunol. 45:868-75 (2008).
U-CyTech products used in this study:
Mouse IFN-γ ELISPOT kit
Mouse IL-4 ELISPOT kit

Chea S., Dale C.J., De Rose R., Ramshaw I.A. and Kent S.J.
Enhanced cellular immunity in macaques following a novel peptide immunotherapy.
J Virol 79:3748-57 (2005).
U-CyTech products used in this study:
Monkey IFN-γ ELISPOT kit
Monkey species: Macaca nemestrina

Chen L., Zhang Z., Chen W., Li Y., Shi M., Zhang J., Wang S., and Wang F.S.
B7-H1 up-regulation on myeloid dendritic cells significantly suppresses T cell immune function in patients with chronic hepatitis.
B. J Immunol 178:6634-41 (2007).
U-CyTech products used in this study:
Human IFN-γ ELISPOT kit

Hamano T., Matsuo K., Hibi Y., Victoriano A.F., Takahashi N., Mabuchi Y., Soji T., Irie S., Sawanpanyalert P., Yanai H., Hara T., Yamazaki S., Yamamoto N., and Okamoto T.
A single-nucleotide synonymous mutation in the gag gene controlling human immunodeficiency virus type 1 virion production.
J Virol 81:1528-33 (2007).
U-CyTech products used in this study:
Human IFN-γ ELISPOT kit

Herath S., Benlahrech A., Papagatsias T., Athanasopoulos T., Bouzeboudjen Z., Hervouet C., Klavinskis L., Meiser A., Kelleher P., Dickson G. and Patterson S.
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
PLoS ONE 9: e88327 (2014)
U-CyTech products used in this study:
Mouse IFN-γ ELISPOT kit

Skowera A., de Jong E.C., Schuitemaker J.H., Allen J.S., Wessely S.C., Griffiths G., Kapsenberg M., and Peakman M.
Analysis of anthrax and plague biowarfare vaccine interactions with human monocyte-derived dendritic cells.
J Immunol 175:7235-43 (2005).
U-CyTech products used in this study:
Human IFN-γ ELISPOT kit
Human IL-2 ELISPOT kit
Human IL-4 ELISPOT kit
Human IL-13 ELISPOT kit

Stittelaar K.J., Neyts J., Naesens L., van Amerongen G., van Lavieren, R.F., Holy A., De Clercq E., Niesters H.G., Fries E., Maas C., Mulder P.G., van der Zeijst B.A., and Osterhaus A.D.
Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infection.
Nature 439:745-8 (2006).
U-CyTech products used in this study:
Monkey IFN-γ ELISPOT kit
Monkey species: Macaca fascicularis

Verstrepen B.E., Bins A.D., Rollier C.S., Mooij P., Koopman G., Sheppard N.C., Sattentau Q., Wagner R., Wolf H., Schumacher T. N., Heeney J.L., and Haanen J.B.
Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates. Vaccine 26:3346-51 (2008).
U-CyTech products used in this study:
Monkey IFN-γ ELISPOT kit
Monkey IL-2 ELISPOT kit
Monkey IL-4 ELISPOT kit
Monkey species: Macaca mulatta

Zhang W., Ahmad G., Torben W., Noor Z., Le L., Damian R.T., Wolf R.F., White G.L., Chavez-Suarez M., Podesta R.B., Kennedy R.C., and Siddiqui A.A.
Sm-p80-based DNA vaccine provides baboons with levels of protection against Schistosoma mansoni infection comparable to those achieved by the irradiated cercarial vaccine.
J Infect Dis 201:1105-12 (2010).
U-CyTech products used in this study:
Monkey IFN-γ ELISPOT kit
Monkey IL-4 ELISPOT kit
Monkey species: Papio anubis